Selected Publications

Scholey, J.M., B. Neighbors, J.R. McIntosh and E.D. Salmon. (1984) Isolation of microtubules and a dynein-like MgATPase from unfertilized sea urchin eggs. J. Biol. Chem. 259: 6516-6525.

Scholey, J.M., M.E. Porter, P.M. Grissom and J.R. McIntosh. (1985) Identification of kinesin in sea urchin eggs, and evidence for its localization in the mitotic spindle. Nature 318: 483-486.

Porter, M.E., J.M. Scholey, D.L. Stemple, G.P.A. Vigers, R.D. Vale, M.P. Sheetz and J.R. McIntosh. (1987) Characterization of the microtubule movement produced by sea urchin egg kinesin. J. Biol. Chem. 262: 2794-2802.

Leslie, R.J., R.B. Hird, L. Wilson, J.R. McIntosh and J.M. Scholey. (1987) Kinesin is associated with a nonmicrotubule component of sea urchin mitotic spindles. Proc. Natl. Acad. Sci. (USA) 84: 2771-2775.

Lye, R.J., M.E. Porter, J.M. Scholey and J.R. McIntosh. (1987) Identification of a microtubule-based cytoplasmic motor in the nematode C. elegans. Cell 51: 309-318.

Cohn, S.A., A.L. Ingold and J.M. Scholey. (1987) Correlation between the ATPase and microtubule translocating activities of sea urchin egg kinesin. Nature 328: 160-163.

Saxton, W.M., M.E. Porter, S.A. Cohn, J.M. Scholey, E.C. Raff and J.R. McIntosh. (1988) Drosophila kinesin: Characterization of microtubule motility and ATPase. Proc. Natl. Acad. Sci. (USA) 85: 1109-1113.

Ingold, A.L., S.A. Cohn and J.M. Scholey. (1988) Inhibition of kinesin-driven microtubule motility by monoclonal antibodies to kinesin heavy chains. J. Cell Biol. 107: 2657-2667.

Cohn, S.A., A.L. Ingold and J.M. Scholey. (1989) Quantitative analysis of sea urchin egg kinesin-driven microtubule motility. J. Biol. Chem. 264: 4290-4297.

Scholey, J.M., J. Heuser, J.T. Yang and L.S.B. Goldstein. (1989) Identification of globular mechanochemical heads of kinesin. Nature338: 355-357.

Wright, B.D., J.H. Henson, K.P. Wedaman, P.J. Willy, J.N. Morand and J.M. Scholey. (1991) Subcellular localization and sequence of a sea urchin kinesin heavy chain: Evidence for its association with membranes in the mitotic apparatus and interphase cytoplasm. J. Cell Biol.113: 817-833.

Cole, D.G., W.Z. Cande, R.J. Baskin, D.A. Skoufias, C.J. Hogan and J.M. Scholey. (1992) Isolation of a sea urchin egg kinesin-related protein using peptide antibodies. J. Cell Science 101: 291-301.

Wedaman, K.P., A.E. Knight, J. Kendrick-Jones and J.M. Scholey. (1993) Sequences of sea urchin kinesin light chain isoforms. J. Mol. Biol. 231: 155-158.

Hogan, C.J., H. Wein, L. Wordeman, J.M. Scholey, K.E. Sawin and W.Z. Cande. (1993) Inhibition of anaphase spindle elongation in vitro by a peptide antibody that recognizes kinesin motor domain. Proc. Natl. Acad. Sci. (USA) 90: 6611-6615.

Wright, B.D., M. Terasaki and J.M. Scholey. (1993) Roles of kinesin and kinesin-like proteins in sea urchin early embryonic cell divisions: Evaluation using antibody microinjection. J. Cell Biol. 123: 681-689.

Cole, D.G., D.S. Chinn, K.P. Wedaman, K. Hall, T. Vuong and J.M. Scholey. (1993) Novel heterotrimeric kinesin related protein purified from sea urchin eggs. Nature 366: 268-270.

Skoufias, D.A., D.G. Cole, K. P. Wedaman and J.M. Scholey. (1994) The carboxyl-terminal domain of kinesin heavy chain is important for membrane binding. J. Biol. Chem. 269: 1477-1485.

Cole, D.G., W.M. Saxton, K.B. Sheehan and J.M. Scholey. (1994) A slow homotetrameric kinesin-related motor protein purified from Drosophila embryos. J. Biol. Chem.269: 22913-22916.

Rashid, D.J., K.P. Wedaman and J.M. Scholey. (1995) Heterodimerization of the two motor subunits of the heterotrimeric kinesin, KRP 85/95. J. Mol. Biol. 252: 157-162.

Henson, J.H., D.G. Cole, M. Terasaki, D.J. Rashid and J.M. Scholey. (1995) Immunolocalization of the hetrotrimeric kinesin-like protein, KRP 85/95 to the mitotic apparatus of sea urchin embryos. Dev. Biol. 171(1): 182-194

Kashina, A.S., R.J. Baskin, D.G. Cole, K.P. Wedaman, W.M. Saxon and J.M. Scholey. (1996) A bipolar kinesin. Nature 379: 270-272.

Scholey, J.M. (1996) Kinesin-II, A Membrane Traffic Motor in Axons, Axonemes and Spindles. J Cell Biol. 133:1-4

Kashina, A.S., J.M. Scholey, J.D. Leszyk and W.M. Saxton. (1996) An essential bipolar mitotic motor. Nature 384:225.

Wedaman, K.P., D.W. Meyer, D.J. Rashid, D.G. Cole and J.M. Scholey. (1996) Sequence and submolecular localization of the 115-kD accessory subunit of the heterotrimeric kinesin-II (KRP85/95) complex. J. Cell Biol. 132(3):371-380.

Bi, G-Q., R.L. Morris, G. Liao, J.M. Alderton, J.M. Scholey and R.A. Steinhardt. (1997) Kinesin- and myosin-driven steps of vesicle recruitment for CA2+-regulated exocytosis. J. Cell Biol. 138(5):999-1008.

Morris, R.L. and J.M. Scholey. (1997) Heterotrimeric kinesin-II is required for the assembly of motile 9+2 ciliary axonemes on sea urchin embryos. J. Cell Biol. 138(5):1009-1022.

Sharp, D. J., K.L. McDonald, H.M. Brown, H.J. Matthies, C. Walczak, R.D. Vale, T.J. Mitchison and J.M. Scholey. (1999) The bipolar kinesin, KLP61F, cross-links microtubules within interpolar microtubule bundles of Drosophila embryonic mitotic spindles. J. Cell Biol. 144: 125-138.

Signor, D., K.P. Wedaman, L.S. Rose and J.M. Scholey. (1999) Two heteromeric kinesin complexes in chemosensory neurons and sensory cilia of Caenorhabditis elegans. Mol. Biol. Cell 10:345-360.

Orozco, J., K.P. Wedaman, D. Signor, H. Brown, L.S. Rose and J.M. Scholey. (1999) Movement of motor and cargo along cilia. Nature 398:674.

Sharp, D.J., C. Yu, J. Sisson, W. Sullivan and J.M. Scholey. (1999) Antagonistic microtubule sliding motors position mitotic centrosomes in Drosophila embryos. Nature Cell Biol. 1:50-54.

Signor, D., K.P. Wedaman, J.T. Orozco, N.D. Dwyer, C. I. Bargmann, L.S. Rose, and J.M. Scholey. (1999). Role of a class DHC1b dynein in retrograde transport of IFT motors and IFT raft particles along cilia, but not dendrites, in chemosensory neurons of living Caenorhabditis elegans. J. Cell Biol.. 147:519-530.

Signor, D. and J.M. Scholey. (2000) Microtubule-based transport in axons, dendrites and axonemes. Essays in Biochemistry. 35: 89-102.

Sharp, D.J., H. Brown, M. Kwon, G.C. Rogers, G. Holland and J.M. Scholey. (2000) Functional coordination of three mitotic motors in Drosophila embryos. Mol. Biol. Cell. 11:241-253.

Sharp, D.J., G.C. Rogers and J.M. Scholey. (2000) Cytoplasmic dynein is required for poleward chromosome movement during mitosis in Drosophila embryos. Nature Cell Biology.2: 922-930.

Sharp, D.J., G.C. Rogers and J.M. Scholey. (2000) Microtubule-motors in mitosis. Nature.407: 41-47. (Commissioned review article)

Signor, D., Rose, L.S., and J.M. Scholey. (2000) Analysis of the roles of kinesin and dynein motors in microtubule-based transport in the C.elegans nervous system. Methods 22:317-325.

Zhou, H.M., Brust-Mascher, I., and J.M. Scholey. (2001) Direct visualization of the movement of the monomeric axonal transport motor, UNC-104, along neuronal processes in living C. elegans. J. Neurosci. 21:3749-3755.

Scholey, J.M., Rogers, G.C., and Sharp, D.J. (2001) Mitosis, microtubules, and  the matrix. J. Cell Biol. 154: 261-266.

Brust-Mascher, I., Scholey J.M. (2002) Microtubule flux and sliding in mitotic spindles of Drosophila embryos. Mol. Biol. Cell. 13: 3967-3975.

Cytrynbaum, E.N., Scholey, J.M., and A. Mogilner. (2003) A force balance model of early spindle pole separation in Drosophila embryos. Biophys. J.. 84: 757-769.

Scholey, J.M., Brust-Mascher, I., Mogilner A. (2003) Cell division. Nature. 422: 746-52. Review.

Scholey, J.M. (2003) Intraflagellar Transport. Ann. Rev. Cell. Dev. Biol. 19: 423-443.

Kwon, M.J., S. Morales-Mulia, I. Brust-Mascher, G.C. Rogers, D.J. Sharp, and J.M. Scholey. (2004) The chromokinesin, KLP3A, drives spindle pole separation during prometaphase and anaphase, and facilitates chromatid motility. Mol. Biol. Cell 15(1): 219-33.

Rogers GC, Rogers SL, Schwimmer TA, Ems-McClung SC, Walczak CE, Vale RD, Scholey JM, Sharp DJ. (2004) Two mitotic kinesins cooperate to drive sister chromatid separation during anaphase. Nature. 427(6972):364-70.

Lawrence, C.J., Dawe, R.K., Christie, K.R., Cleveland, D.W., Dawson, S.C., Endow, S.A., Goldstein, L.S.B., Goodson, H.V., Hirokawa, N., Howard, J., Malmbert, R.L., McIntosh, J.R., Miki, H., Mitchison, T.J., Okada, Y., Reddy, A.S.N. Saxton, W.M., Schliwa, M., Scholey, J.M., Vale, R.D., Walczak, C.E. and Wordeman, L. (2004) A Standardized Kinesin Nomenclature. J. Cell Biol. 167: 19-22.

Snow, J, Ou, G, Gunnarson, A, Walker, R, Zhou, HM, Brust-Mascher, I and Scholey JM. (2004) Two Anterograde Intraflagellar Transport Motors Cooperate to Build Sensory Cilia on C. elegans Neurons. Nature Cell Biol. 6 (11):1109-1113

Brust-Mascher, I., Civelekoglu-Scholey, G., Kwon, M., Mogilner, A., and Scholey, J.M. (2004) Model for Anaphase B: Role of three mitotic motors in a switch from poleward flux to spindle elongation. Proc. Natl. Acad. Sci (USA). 101 (45) 15938-15943.

Guangshuo Ou, Oliver E. Blacque, Joshua J. Snow, Michel R. Leroux & Jonathan M. Scholey. (2005) Functional coordination of intraflagellar transport motors. Nature Jul 28;436(7050):583-7.

Civelekoglu-Scholey, G., Sharp, DJ, Mogilner, A., and Scholey, J.M. (2006) Model of chromosome motility in Drosophila embryos: adaptation of a general mechanism for rapid mitosis. Biophys. J. 90(11):3966-82.

Pan, X., Ou, G., Civelekoglu-Scholey, G.,Blaque O.E., Endres N.F., Tao, L, Mogilner, A., Leroux, M.R., Vale, R., and Scholey, J.M. (2006) Mechanism of transport of IFT particles in C. elegans cilia by the concerted action of kinesin-II and OSM-3 motors. J. Cell Biol. 174(7):1035-45.

Tao L, Mogilner A, Civelekoglu-Scholey G, Wollman R, Evans J, Stahlberg H, Scholey JM. (2006) A Homotetrameric Kinesin-5, KLP61F, Bundles Microtubules and Antagonizes Ncd in Motility Assays. Curr. Biol. 16(23): 2293-302.

Cheerambathur DK, G Civelekoglu-Scholey, I Brust-Mascher, P Sommi, A Mogilner, and J.M. Scholey. (2007) Quantitative analysis of an anaphase B switch: predicted role for a microtubule catastrophe gradient. J Cell Biol. 177: 995-1004.

Civelekoglu-Scholey G and J.M. Scholey. (2007) Mitotic Motors: Kinesin-5 Takes a Brake. Curr. Biol. 17: R544-R547.

Brust-Mascher, I and JM Scholey. (2007) Mitotic Spindle Dynamics in Drosophila. Int. Rev. of Cytology, 259: 139-172.

Wollman R, Civelekoglu-Scholey G, Scholey JM and A Mogilner. (2008) Reverse engineering of force integration during mitosis in the Drosophila embryo. Mol. Systems Biol. 4:195.

Cheerambathur DK, I Brust-Mascher, G Civelekoglu-Scholey, and J.M. Scholey. (2008) Dynamic partitioning of mitotic kinesin-5 cross-linkers between microtubule-bound and freely diffusing states. J Cell Biol. 182: 429-426.

van den Wildenberg, SMJL, L Tao, LC Kapitein, CF Schmidt, JM Scholey, and EJG Peterman. (2008) The Homotetrameric Kinesin-5 KLP61F Preferentially Crosslinks Microtubules into Antiparallel Orientations. Curr. Biol. 18: 1860–1864.

Brust-Mascher, I, P. Sommi, D.K. Cheerambathur, and J.M. Scholey (2009) Kinesin-5-dependent poleward flux and spindle length control in Drosophila embryo mitosis. Mol. Biol. Cell 20: 1749-1762.

Brust-Mascher, I and JM Scholey. (2009) Microinjection Techniques for Studying Mitosis in the Drosophila melanogaster Syncytial Embryo. JoVE. 31. doi: 10.3791/1382

Civelekoglu-Scholey G, L Tao, I Brust-Mascher, R Wollman, and J.M. Scholey. (2010) Prometaphase spindle maintenance by an antagonistic motor-dependent force balance made robust by a disassembling lamin-B envelope. J Cell Biol. 188: 49-68.

Wang, H, I Brust-Mascher, D Cheerambathur and J.M. Scholey. (2010) Coupling Between Microtubule Sliding, Plus-End Growth and Spindle Length Revealed by Kinesin-8 Depletion. Cytoskeleton 67: 715-728.

Goshima, G. and J.M. Scholey. (2010) Control of Mitotic Spindle Length. Annual Rev. of Cell and Dev. Biol. 26: 21-57.

Hao, L., M. Thein, I. Brust-Mascher, G. Civelekoglu-Scholey, Y. Lu, S. Acar, B. Prevo, S. Shaham, and J.M. Scholey. (2011) Intraflagellar transport delivers tubulin isotypes to sensory cilium middle and distal segments. Nature Cell Biol. 13(7):790-8 doi: 10.1038/ ncb2268.

Acar, S., D.B. Carlson, M.S. Budamagunta, V. Yarov-Yarovoy, J.J. Correia, M.R. Ninonuevo, W. Jia, L. Tao, J.A. Leary, J.C. Voss, J.E. Evans and J.M. Scholey. (2013) The bipolar assembly domain of the mitotic motor kinesin-5. Nature Communications 4:1342 doi: 10.1038/ncomms2348.

Scholey, J.M. (2013) Kinesin-2: A family of heterotrimeric and homodimeric motors with diverse intracellular transport functions. Annual Review of Cell and Developmental Biology. 29: 44369.

Wang, H., Brust-Mascher I, Civelekoglu-Scholey G and Scholey JM. (2013) Patronin Mediates a switch from Kinesin-13-dependent Poleward Flux to Anaphase B Spindle Elongation. J. Cell Biology. 203, 35-46.

Scholey J.E., Nithianantham S., Scholey J.M. and Al-Bassam J. (2014) Structural basis for the assembly of the mitotic motor Kinesin-5 into bipolar tetramers. eLife 3:e02217.

Wang, H., Brust-Mascher I, and Scholey JM. (2015) The microtubule cross-linker Feo, controls the midzone stability, motor composition, and elongation of the anaphase B spindle in Drosophila embryos. Mol. Biol. Cell. 26, 1456-52.

Brust-Mascher I, Civelekoglu-Scholey G and Scholey JM. (2015) Mechanism for Anaphase B: Evaluation of “Slide-and-Cluster” versus “Slide-and-Flux-or-Elongate” Models. Biophys. J. 108, 2007-18.

Prevo B, Mangeol P, Oswald F, Scholey JM, Peterman EJ. (2016) Functional differentiation of cooperating kinesin-2 motors orchestrates cargo import and transport in C. elegans cilia. Nature Cell Biol. 17(12):1536-45.